Product Description
3M 1296F - Attest Rapid Readout Biological Indicator Test Pack, 250F/ 121C Gravity or 270F/ 132C Vacuum Assisted Sterilizers, Includes 25 Test Packs & 5 Controls, 3 Hour Readout, Brown Cap, 25/CS
3M 1296F Attest Rapid Readout Biological Indicator Test Pack
The 3M Attest Rapid Steam Biological Indicator Test Pack 1296/1296F is a Biological Indicator (BI) test pack for 250?/121? gravity displacement and 270F/132? vacuum-assisted steam sterilization cycles. Each pack contains one (1) 3M Attest Rapid Steam Biological Indicator, 1292, Brown Cap, 3 Hour. This product must be used with the 3M Attest Auto-reader 390.
The 3M Attest Rapid Steam Biological Indicator Test Pack, 1296/1296F, presents a resistant challenge to the steam sterilization process equivalent to the AAMI 16-towel PCD. This biological indicator (BI) test pack is used to monitor 250?/121? gravity displacement steam sterilization cycles of 40 minutes and 270?/132? vacuum-assisted steam sterilization cycles of 4 minutes. Each pack contains one (1) 3M Attest Rapid Steam Biological Indicator, 1292, Brown Cap, 3 Hour, and must be used with the 3M Attest Auto-reader 390. The BI result is available after 3 hours of incubation in a 3M Attest Auto-reader 390.
Features and Benefits of 3M 1296F Attest Rapid Readout Biological Indicator Test Pack
- Pre-assembled, easy-to-use test pack containing a 3M Attest Rapid Readout Biological Indicator 1292, Brown Cap, 3 Hour
- Accurate BI results in 3 hours when incubated in a 3M Attest Auto-reader 390
- Cost and time-efficient readouts
- Test packs present a challenge to the sterilization process equivalent to the AAMI 16-towel PCD
- Interested in a faster BI result? Learn more about 3M Attest Super Rapid Steam Biological Indicator Challenge Pack 51582
3M 1296F Attest Rapid Readout Biological Indicator Test Pack Directions for Use
- Place a 3M Attest 1296 Rapid Readout Biological Indicator Steam Pack, with the label side up, in a full load in the most challenging area for the sterilant. This is generally on the bottom shelf, near the door and over the drain.
- Process the load according to recommended practices.
- After completion of the cycle, while wearing heat resistant gloves, retrieve the 3M Attest 1296 Rapid Readout Biological Indicator Steam Pack.
- Check to see that the external process indicator on the outside of the test pack has turned from yellow to brown or darker. Then open the pack for 5 minutes to dissipate heat prior to removing the biological indicator. Next, allow the biological indicator to cool outside the pack for an additional 10 minutes prior to crushing.
- Remove the record keeping sheet from inside the 3M&trade Attest&trade 1296 Rapid Readout Biological Indicator Steam Pack. Check the chemical indicator on the record keeping sheet. A color change from yellow to brown or darker confirms the pack has been exposed to the steam sterilization process. This color change does not indicate that the process was sufficient to achieve sterility. For a permanent record, fill out the required information on the record keeping sheet. Record the biological indicator result when it is available.
- Slip the coil off the Attest biological indicator.
- Check the throughput chemical indicator (CI) on the label of the Attest 1292 Rapid Readout Biological Indicator. A color change from rose to brown/black confirms that the biological indicator has been exposed to the steam sterilization process. This color change does not indicate that the process was sufficient to achieve sterility.
- Identify the Attest biological indicator by writing the sterilizer, load number, and processing date on the indicator label. Do not place another label or indicator tape on the Attest biological indicator vial.
- Discard the test pack. Using a test pack more than once will invalidate subsequent test results.
- While wearing safety glasses, press the biological indicator cap down. Crush the glass ampoule of the biological indicator in the crusher well of the auto-reader. Hold the biological indicator by the cap and tap on a hard surface, but not on the auto-reader, until the media wets the strip at bottom of the vial. Then place the biological indicator in an auto-reader incubation well configured to incubate Attest 1292 Rapid Readout Biological Indicators. See Attest Auto-reader Operators Manual for further details.
- Each day that a processed Attest 1292 Rapid Readout Biological Indicator is incubated, crush, tap and incubate at least one non-processed Attest 1292 Rapid Readout Biological Indicator to use as a positive control. Write a C (for control) and the date on the label. The positive control biological indicator should be from the same manufacturing date and lot number as the processed biological indicator. It is a good practice to incubate a positive control for a visual color change each day a processed biological tested is incubated. This helps ensure:
- correct incubation temperatures are met,
- viability of spores have not been altered due to improper storage temperature, humidity or proximity to chemicals,
- capability of media to promote rapid growth, and
- proper functioning of Attest Auto-reader
Interpretation of Results:
Fluorescent Result
The positive (unprocessed) control biological indicator must provide a positive result [red light on the 3M Attest 290 Auto-reader or plus symbol (+) on the LCD display of the 3M Attest Auto-reader 390]. If the positive biological indicator control reads negative [green light on the 3M Attest 290 Auto-reader or minus symbol (-) on the LCD display of the 3M Attest Auto-reader 390], refer to the applicable auto-reader Operators Manual Troubleshooting section. Retest the auto-reader with a new positive control biological indicator. The processed biological indicator results are not valid until the positive biological indicator control reads positive. With a processed biological indicator, a positive [red light on the 3M Attest 290 Auto-reader or plus symbol (+) on the LCD display of the 3M Attest Auto-reader 390] means a sterilization process failure has occurred. A negative [green light on the 3M Attest 290 Auto-reader or minus symbol (-) on the LCD display of the 3M Attest Auto-reader 390] after 3 hours of incubation indicates an acceptable sterilization process.
pH Color Change Result (Optional)
The appearance of a yellow color in the processed indicator demonstrates bacterial growth and a sterilization process failure. No color change (i.e. media remains purple) indicates an adequate sterilization process. A final negative result is made after 48 hours of incubation. The positive control indicator should show a color change from purple to yellow for the processed indicator results to be valid.
- Record the processed and control biological indicator results. Act on any positive test result as soon as it is noted. Always retest the sterilizer and do not use the sterilizer until three consecutive biological indicator results are negative.
3M 1296F Attest Rapid Readout Biological Indicator Test Pack Specifications
Biological Indicator System | Rapid |
Brands | Attest |
Cap Colour | Brown |
Catalog Number | 1296F |
Clinical Area | Medical Device Reprocessing Department |
Incubation Period | 3 hr |
Inner Packs per Case | 0 |
Product Type | Biological Indicator |
Product Use | Load Monitoring |
Sterilization Method | Steam |
Total Units per Case | 25 per Case |
Units per Inner Pack | 0 |
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