Lifesign 1003 - Uricult CLED+Polymyxin/MacConkey, 10/BX
Uricult CLED+Polymyxin/MacConkey (Urinary Tract Infections)
10 Urine Culture-Paddles for the detection of bacteriuria and the presumptive identification of uropathogens. The test is intended for in vitro diagnostic use.
- Moderately Complex
- Culture method for urinary pathogens
- Easy to perform procedure; Dip - Incubate - Read Results
- Quick and convenient result within 24 hours
- Simple CLIA compliance for reporting colony counts.
Uricult CLED+Polymyxin/MacConkey Certificate of Analysis
| pH range | Growth results | CLED | MC | Lactose reaction |
| CLED+Pol 6.97.4 | Escherichia coli - ATCC 25922 | Inhibited | Growth | Pos. |
| Proteus mirabilis - ATCC 12453 | Growth | Growth | Neg. | |
| Proteus vulgaris - ATCC 8427 | Growth | Growth | Neg. | |
| MacConkey 6.97.4 | Salmonella typhimurium - ATCC 14028 | Inhibited | Growth | Neg. |
| Enterococcus faecalis - ATCC 29212 | Growth | No growth | Pos. | |
| Staphylococcus aureus - ATCC 25923 | Growth | No growth | Pos. |
Summary and explanation of the test
In the mid 1960's, Mackey and Sandys developed a dip inoculum transport medium for rapid urine culturing . Subsequent improvements have been made which increase the accuracy of the results obtained while retaining the convenience of the dip inoculum technique.
URICULT Urine CULTURE-PADDLES provide effective bacterial detection and presumptive identification in a simple and reliable manner.
URICULT Urine CULTURE-PADDLES are attached to a screw cap. Each side of the culture-paddle is coated with an agar medium suitable for the growth of urinary bacteria, and the culture-paddle is suspended in a clear plastic vial.
The CULTURE-PADDLES are safely isolated in this vial during transport, incubation, storage and handling. Because of the uniform application of agar to the URICULT Urine CULTUREPADDLE, it is possible to obtain semi-quantitative results when the device is used as directed. This is determined by a simple visual comparison of bacterial growth on the agar surface with the Colony Density Chart provided. No actual colony counting is necessary
Principles of the procedure
CLED-Polymyxin Agar
CLED medium was first described by Mackey and Sandys1 specifically for use in dip inoculum procedures for urinary bacteriology. The electrolyte deficient nature of the medium prevents the characteristics swarming of Proteus. The inclusion of lactose and the indicator dye, Brom-Thymol Blue, allows differentiation of lactose fermenting bacteria, since the by-products of lactose fermentation cause the color of the agar to change from its original pale green color toward yellow.
The incorporation of Polymyxin into the CLED medium creates a selective medium for the growth of Enterococci, Staphylococci, Proteus, and Yeast, preventing the growth of most E. coli, Klebsiella, and Enterobacter. Additionally, Pseudomonas colony growth is inhibited. Occasionally, mutant colonies of E. coli, Klebsiella, and Enterobacter will be observed on this medium as large mucoid colonies.
MacConkey Agar
The URICULT MacConkey agar is selective for the growth of Gram negative bacteria, and is inhibitory to most Gram positive organisms. The neutral red indicator included in the formulation causes the colonies of lactose fermenting bacteria to appear pink or red.
Reagents
| Uricult CLED+Polymyxin/ MacConkey | Product No. 1003 |
| Urine CULTURE-PADDLES (in individual vials) | 10 |
| Patient labels | 10 |
| Instructions for use containing the Colony Density Chart | 1 |
Typical formulation
| CLED-Polymyxin medium | MacConkey medium |
| Peptone 10.0 g/L | Peptone 20.0 g/L |
| Meat extract 3.0 g/L | Lactose 10.0 g/L |
| Lactose 10.0 g/L | Bile Salts Mixture |
| L-Cystine 0.13 g/L | Neutral Red |
| Brom-Thymol Blue | Crystal Violet |
| Polymyxin |
Uricult CLED+Polymyxin/MacConkey Density Chart
Procedure
Compliance with the following directions is required to achieve reliable test results.
